NOVEL REAGENTS FOR CREATINE KINASE
نویسندگان
چکیده مقاله:
The purpose of this investigation was to develop a simple colorimetric method for creatine kinase (CK). The new method is based on the reaction of creatine, formed enzymatically from creatine phosphate and ADP, with different glyoxal compounds. Hydrated glyoxals, such as para-nitrophenyl, 2-thiophene, 4- biphenyl, 4, 4' -biphenyl,α-naphthyl, β-naphthyl, para-chlorophenyl, and styryl were synthesized and allowed to react with creatine. Among the glyoxals, the 2- thiophene derivative was the best in terms of the stability and intensity of the colored complex which was produced under mild alkaline conditions. The complex absorbed maximally at 460 nm with an extinction coefficient of 1.56x 1 04 M-I Cm-I. This reagent was used to determine CK in the sera of normal human beings and patients with myocardial infarction. The results obtained were in agreement with those obtained by another available method for CK. However, this new method is simple, less time consuming and employs a single reagent for color development. Such a simple method might be of value in clinical laboratories with little access to sophisticated instruments such as auto analyzers and spectrophotometers
منابع مشابه
novel reagents for creatine kinase
the purpose of this investigation was to develop a simple colorimetric method for creatine kinase (ck). the new method is based on the reaction of creatine, formed enzymatically from creatine phosphate and adp, with different glyoxal compounds. hydrated glyoxals, such as para-nitrophenyl, 2-thiophene, 4- biphenyl, 4, 4' -biphenyl,α-naphthyl, β-naphthyl, para-chlorophenyl, and styryl were s...
متن کاملCreatine and creatine kinase measurement.
*Many methods for measuring creatine are known, the principal ones being direct calorimetric assay (l), indirect calorimetric assay (2), and destruction with bacterial adaptive enzymes (3). However, none of these methods are specific nor are they sensitive enough to detect very small amounts of creatine. rIhese assays have been used to examine urine and serum for creatine content, but, because ...
متن کاملAn interlaboratory study of creatine kinase and creatine kinase isoenzymes.
Over 400 laboratories participated in an interlaboratory study of creatine kinase (EC 2.7.3.2) in which they were requested to analyze five lyophilized samples for total creatine kinase and creatine kinase isoenzymes and to complete a questionnaire regarding methodology. The specimens were prepared from an homogenate of human myocardium that had been centrifuged, dialyzed, and added to a matrix...
متن کاملCreatine Kinase Activity and Cartilage Energy Metabolism: Creatine Kinase-creatine Phosphate Circuit Activity and Macromolecule Synthesis
Earlier studies showed that there is significant creatine kinase activity in the epiphyseal growth cartilage, and there is stage-specific isoform expression. To further explore the role of this enzyme in cartilage development, we disrupted the creatine kinase creatine phosphate (CK-CP) circuit using the creatine analogue, cyclocreatine. We found that while cyclocreatine decreased creatine kinas...
متن کاملLevels of C-reactive protein, creatine kinase-muscle and aldolase A are suitable biomarkers to detect the risk factors for osteoarthritic disorders: A novel diagnostic protocol
Background: C-reactive protein (CRP), creatine kinase-muscle (CK-MM) and aldolase A (AldoA) levels are predicted to be realistic biomarkers of osteoarthritic disorders (OADs). The objective of the study was to evaluate the levels of CRP, CK-MM, and AldoA and determine their correlations with risk factors such as inflammation, muscle degeneration, and skeletal muscle damage for OADs. Methods: B...
متن کاملเวชศาสตร์ร่วมสมัย Macro creatine kinase
Macro creatine kinase (Macro CK), a macro enzyme, is a major cause of misdiagnosis of myocardial infarction upon determination of total creatine kinase and creatine kinase isoenzyme levels. It presents in elderly female patients with an approximate prevalence of 1 %. Based on composition, there are two major types of macro CK Type 1 macro CK is a complex of Type 1 being an immune complex of cre...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ذخیره در منابع من قبلا به منابع من ذحیره شده{@ msg_add @}
عنوان ژورنال
دوره 10 شماره 4
صفحات 299- 304
تاریخ انتشار 1997-02
با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023